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iris and pupil detection

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Hello everyone, I want to localize the iris and pupil in eye image. I used the circle how transforms of opencv, but I can't estimate the correct parameters' values. If there is any way to correctly estimation parameters, please tell me how?? Or Are there any mistake in the following my code? the original image : [C:\fakepath\img_10_1_1.jpg](/upfiles/14471394799364387.jpg) canny image: [C:\fakepath\20151109232057.jpg](/upfiles/14471401584037078.jpg) and I didn't get any circle. the code: //convert image to gray org.opencv.imgproc.Imgproc.cvtColor(mRgba, imgCny, Imgproc.COLOR_RGB2GRAY); //apply threshold and morphology org.opencv.imgproc.Imgproc.threshold(imgCny, imgCny, 150, 150, Imgproc.THRESH_BINARY); org.opencv.imgproc.Imgproc.morphologyEx(imgCny, imgCny, Imgproc.MORPH_CLOSE,Imgproc.getStructuringElement(Imgproc.MORPH_RECT, new Size(3, 3))); org.opencv.imgproc.Imgproc.morphologyEx(imgCny,imgCny,Imgproc.MORPH_ERODE ,Imgproc.getStructuringElement(Imgproc.MORPH_RECT,new Size(3,3) )); //canny org.opencv.imgproc.Imgproc.Canny(imgCny, imgCny, 10,30 ); //Hough Circles org.opencv.imgproc.Imgproc.HoughCircles(imgCny,circles, Imgproc.CV_HOUGH_GRADIENT,1,imgCny.rows()/8,100,300,2,400); //draw circle if(!circles.empty()){ for (int i=0; i < circles.cols();i++){ double vCircle[] = circles.get(0,i); pt = new Point((int) Math.round((vCircle[0])),(int) Math.round((vCircle[1]))); int radius = (int) Math.round(vCircle[2] ); Core.circle(mRgba, pt, radius, new Scalar(0,255,0),3);

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