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how to crop ROI?

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Hello, I have already set ROI for my image and now I want to crop ROI again. for example as shown in figure, there is already ROI and the read box which I want cropping. ![image description](/upfiles/14492456198920777.png) Mat image = roi1(Rect(0, 0, 158, 695)); //ROI for perticulat tubes Rect(0, 76, 158, 545) imshow ("1", Tube1); I want to do so because I am want to use both of image in my operation. Is there any suggestion how to crop the ROI again. Thank you very much in advance.

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