Hello,
I have already set ROI for my image and now I want to crop ROI again.
for example as shown in figure, there is already ROI and the read box which I want cropping.

Mat image = roi1(Rect(0, 0, 158, 695)); //ROI for perticulat tubes Rect(0, 76, 158, 545)
imshow ("1", Tube1);
I want to do so because I am want to use both of image in my operation.
Is there any suggestion how to crop the ROI again.
Thank you very much in advance.
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